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L-Glutathione

L-谷胱甘肽

Company: Axxora
Catalog#: ALX-157-002-G005
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GST-tagged Yeast Protein Purification
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2011-10-05
[Abstract]  Glutation S-transferase (GST) tagging is the most commonly used purification strategy for recombinant protein. It was developed with the goal of preserving the enzymatic activity by utilizing gentle elution condition of the target protein from purification matrix (Poon and Hunt., 1994). The method described here can be applied from single protein to proteome scale purification of recombinant protein from yeast (Zhu et al., 2000; Zhu et al., 2001). [摘要]  Gligation S转移酶(GST)标签是重组蛋白最常用的纯化策略。 它的开发目的是通过利用来自纯化基质的目标蛋白的温和洗脱条件来保持酶活性(Poon和Hunt,1994)。 本文所述的方法可以从单一蛋白质应用于来自酵母的重组蛋白质的蛋白质组规模纯化(Zhu et al。,2000; Zhu et al。,2001)。

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