| Qualitative in vivo Bioluminescence Imaging
|
|
Author:
Date:
2018-09-20
[Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the optimal use of laboratory animal resources, particularly the ethical principle of reduction in animal use, given its non-invasive nature, ensuring that ongoing biological processes can be studied over time in the same animal, without the need to euthanize groups of mice at specific time points. In this protocol, the luciferase imaging technique was developed to study the effect of co-inoculating pericytes (contractile, αSMA+ mesenchymal ...
[摘要] 生物发光成像（BLI）技术是一种在体内实验室动物上进行分子成像的先进方法。 这种强大的技术广泛应用于研究各种生物过程，是实时探索肿瘤生长和转移扩散的理想工具。 该技术确保实验室动物资源的最佳利用，特别是减少动物使用的伦理原则，考虑到其非侵入性，确保可以在同一动物中随时间研究正在进行的生物过程，而无需安乐死 小鼠在特定的时间点。 在该方案中，开发了荧光素酶成像技术以研究共同接种周细胞（收缩性，αSMA + 间充质干细胞样细胞，位于微血管内的细胞）对生长和转移性扩散的影响。 卵巢癌使用侵袭性卵巢癌细胞系-OVCAR-5-作为例子。
【背景】生物发光成像（BLI）的原理是基于相对简单的生化过程的发光特性，即，荧光素酶介导的分子底物荧光素氧化产生光。在癌症研究中，BLI是一种流行的工具（Contag et ...
|
|
|
| 3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells
|
|
Author:
Date:
2018-04-20
[Abstract] Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated spheroid and clinical pathology of ovarian cancer, and that TAMs promote spheroid formation and tumor growth at early stages of transcoelomic metastasis in orthotopic mouse models. We have established an in vitro spheroid formation assay using a 3D co-culture system in which mouse GFP+F4/80+CD206+ TAMs isolated from spheroids of ovarian cancer-bearing donor tomatolysM-cre mice were mixed with ...
[摘要] 卵巢癌相当独特，因为卵巢癌细胞可以通过腹膜腔直接分离和扩散。然而，目前还不清楚癌细胞如何在腹膜中存活并形成球状结构。我们最近报道，肿瘤相关巨噬细胞（TAMs）相关的球状体与卵巢癌的临床病理学之间存在很强的相关性，并且TAMs在原位小鼠模型中促进球体形成和肿瘤生长在转移瘤体转移的早期阶段。我们已经建立了使用3D共培养系统的体外球体形成测定法，其中小鼠GFP + F4 / 80 + CD206 F 从含有卵巢癌的供体番茄lysM-cre小鼠的球状体中分离的TAM与在含有2％基质胶的培养基中的ID8细胞（TAM:ID8比例为1:10）混合并接种到用Matrigel预包被的24孔板上。由于transcoelomic转移也与许多其他癌症，如胰腺癌和结肠癌，TAM介导的球体形成实验将提供一个有用的方法来定义卵巢癌和其他transcoelomic转移癌症的分子机制和治疗目标。
【背景】在美国，卵巢癌（OC）是第二常见的妇科癌症和主要死亡原因（Jemal等人，2009; Siegel等人，2012年） ）。 OC预后不良的主要原因是腹腔内和盆腔广泛植入转移，通常手术无法完全切除。对腹膜转移现象最广泛的解释是肿瘤细胞在延伸到腹膜表面后与原发肿瘤分离，并在腹膜内播种之前通过腹膜液输送到整个腹腔。有人提出，转移瘤的转移过程可分为几个步骤：1）细胞脱落，失巢凋亡的存活和抵抗; ...
|
|
|