| Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
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Author:
Date:
2016-04-05
[Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).
[摘要] 我们在这里提供了详细的协议，用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节（DRG）中。 解离的小鼠DRG可以通过酶消化解离成单个细胞，以获得小鼠感觉神经元的原代培养物，如Khaminets等人（2015）报道的研究中所进行的。
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| Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
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Author:
Date:
2015-01-05
[Abstract] Compared to an in vivo experiment, neuronal cell cultures are immediately accessible to observation and manipulation. In this protocol, we describe a technique to evaluate the cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).
This protocol is similar to "Neuron-enriched Cultures (Method 2)" protocol (Gao, 2011).
[摘要] 与体内实验相比，神经元细胞培养物可立即进行观察和操作。 在该协议中，我们描述了评价金属锰（Mn 2 + ）对海马神经元细胞培养物的细胞毒性的技术。 有趣的是，该方案容易适用于任何类型的原代培养物（例如，皮层神经元）和任何类型的有毒化合物（例如，化学产品）。
此协议类似于"神经元富集培养物（方法2）"方案（Gao，2011） 。
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| Primary Culture of Cortical Neurons
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Author:
Date:
2013-04-20
[Abstract] Primary culture of neurons from cerebral cortex is a popular model to study neuronal function in vitro and to explore the molecular mechanism of neurite outgrowth in the developing and adult central nervous system. This protocol is for preparing a culture of cerebral cortical neurons from postnatal rodent brain (Muramatsu et al., 2012). One day after cell plating, we can observe neurite outgrowth by microscope.
[摘要] 来自大脑皮质的神经元的原代培养是研究体外神经元功能的流行模型，并且研究发育和成人中枢神经系统中神经突生长的分子机制。 该方案用于从产后啮齿动物脑制备大脑皮层神经元的培养物（Muramatsu等人，2012）。 细胞铺板后一天，我们可以通过显微镜观察神经突生长。
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