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Author:
Date:
2015-01-05
[Abstract] Compared to an in vivo experiment, neuronal cell cultures are immediately accessible to observation and manipulation. In this protocol, we describe a technique to evaluate the cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).
This protocol is similar to "Neuron-enriched Cultures (Method 2)" protocol (Gao, 2011).
[摘要] 与体内实验相比，神经元细胞培养物可立即进行观察和操作。 在该协议中，我们描述了评价金属锰（Mn 2 + ）对海马神经元细胞培养物的细胞毒性的技术。 有趣的是，该方案容易适用于任何类型的原代培养物（例如，皮层神经元）和任何类型的有毒化合物（例如，化学产品）。
此协议类似于"神经元富集培养物（方法2）"方案（Gao，2011） 。
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