{{'Search' | translate}}
 

D-Luciferin

D-荧光素

Company: Sigma-Aldrich
Catalog#: L9504
Bio-protocol()
Company-protocol()
Other protocol()

Determination of Luciferase Activity in Arabidopsis seedling
Author:
Date:
2014-06-20
[Abstract]  There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. This assay was originally optimized for analyzing the repression of PHT1;1 promoter in response to arsenate As (V) which can be adapted to study the kinetics of transcriptional regulation of any gene in response to biotic or environmental stimuli measuring Luciferase activity in Arabidopsis thaliana. [摘要]  有不同的直接和间接方法可用于研究植物系统中的基因表达。 在该协议中,我们描述了使用转基因植物表达在磷酸转运蛋白PHT1; 1启动子控制下的荧光素酶基因的修饰表达测定。 该测定最初被优化用于分析响应于砷酸盐As(V)的PHT1; 1启动子的抑制,其可以适于研究响应于测量荧光素酶活性的生物或环境刺激的任何基因的转录调节的动力学。 拟南芥(Arabidopsis thaliana)。

Comments