|
Author:
Date:
2014-10-05
[Abstract] Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the C-N bond in glyphosate (Pedotti et al., 2009). Here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a glyphosate-degrading marine strain of Bacillus cereus (BceGO), we used a bacteriophage T7 lysis-based method for high-throughput screening of oxidase activity and engineered the gene encoding BceGO by directed evolution.
[摘要] 草甘膦，广泛应用于全世界农业的广谱除草剂在莽草酸途径中抑制5-烯醇丙酮酸莽草酸-3-磷酸合酶，并且已经报道甘氨酸氧化酶（GO）能够催化各种胺的氧化脱氨基， 切割草甘膦中的CN键（Pedotti等人，2009）。 在这里，为了改善从草甘膦降解的蜡状芽孢杆菌菌株（BceGO）克隆的甘氨酸氧化酶的催化活性，我们使用基于噬菌体T7裂解的方法， 通过筛选氧化酶活性并通过定向进化改造编码BceGO的基因。
|