| Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
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Author:
Date:
2016-10-05
[Abstract] In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells.
[摘要] 在这些文化模型中，保留了脊髓的正常细胞结构和局部神经元回路，提供了解离的细胞培养物和完全动物研究之间的折衷。 向培养基中加入5-乙炔基-2'-脱氧尿苷（EdU）允许检测增殖细胞。
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| Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
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Author:
Date:
2016-04-05
[Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).
[摘要] 我们在这里提供了详细的协议，用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节（DRG）中。 解离的小鼠DRG可以通过酶消化解离成单个细胞，以获得小鼠感觉神经元的原代培养物，如Khaminets等人（2015）报道的研究中所进行的。
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| Generation of Aβ-specific T cell lines and in vivo Transfer
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Author:
Date:
2014-09-20
[Abstract] Amyloid-β (Aβ)-containing plaques accumulate in the brains of patients with Alzheimer’s disease (AD). Studies in transgenic mice which over-express amyloid precursor protein and presenilin 1 (APP/PS1 mice) have suggested that T cells that infiltrate the brain may influence the development of Aβ plaques and associated cognitive dysfuncation. Active immunization with Aβ peptides and adjuvants has been evaluated as a therapy for AD, based on the premise that it induces Aβ-specific antibodies that may help to clear the Aβ plaques. However, immunization with Aβ peptides and adjuvants also promotes the development of Aβ-specific T cells (McQuillan et al., 2010) and there is evidence that Aβ-specific T cell may influence the development of Aβ plaques and disease progression in AD ...
[摘要] 含有淀粉样蛋白β（Aβ）的斑块积聚在患有阿尔茨海默病（AD）的患者的脑中。过表达淀粉样蛋白前体蛋白和早老蛋白1（APP/PS1小鼠）的转基因小鼠的研究已经表明浸润大脑的T细胞可能影响Aβ斑块和相关认知功能障碍的发展。使用Aβ肽和佐剂的主动免疫已被评估为AD的治疗，基于其诱导可能有助于清除Aβ斑块的Aβ特异性抗体的前提。然而，用Aβ肽和佐剂免疫也促进Aβ特异性T细胞的发展（McQuillan等人，2010），并且有证据表明Aβ特异性T细胞可能影响Aβ斑块的发展和AD患者的疾病进展。在小鼠模型中，分泌IFN-γ（Th1细胞）的Aβ特异性T细胞已显示增强斑块负荷（Browne等人，2013）。已经在体外极化为Th1，Th2，Th17或Treg细胞的Aβ特异性T细胞的过继转移可用于体内检查这些细胞的功能。
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