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Author:
Date:
2014-09-20
[Abstract] Glycosaminoglycans (GAGs) are long unbranched polysaccharides consisting of repeating disaccharide units composed of a hexosamine (glucosamine or galactosamine) and a hexuronic acid (glucuronic or iduronic acid). Depending on the disaccharide unit the GAGs can be organized into five groups: chondroitin sulfate, dermatan sulfate, heparan sulfate, keratan sulfate and hyaluronic acid. The GAGs are heterogeneous molecules with great variability in molecular mass and both sulfation density and pattern. Spectrophotometric assays to measure the GAG content in biological fluids and tissue/cell extracts are valuable tools. The dye 1,9-dimethylmethylene is a thiazine chromotrope agent that presents a change in the absorption spectrum due to the induction of metachromasia when bound to sulfated GAGs ...
[摘要] 糖胺聚糖（GAG）是长的非支链多糖，由由己糖胺（葡糖胺或半乳糖胺）和己糖醛酸（葡糖醛酸或艾杜糖醛酸）组成的重复二糖单元组成。根据二糖单位，GAG可以分为五组：硫酸软骨素，硫酸皮肤素，硫酸乙酰肝素，硫酸角质素和透明质酸。 GAG是具有分子质量和硫酸化密度和模式的大变异性的异质分子。用于测量生物流体和组织/细胞提取物中GAG含量的分光光度测定法是有价值的工具。染料1,9-二甲基亚甲基是噻嗪变体试剂，其由于当与硫酸化GAG结合时诱导变态变化而呈现吸收光谱的变化，使得能够快速检测溶液中的GAG（Whitley等人， ，1989; Chandrasekhar et al。，1987; Farndale et al。，1982）。此外，存在其中可绘制线性曲线（大约在0.5-5μgGAG之间）的窗口，使得能够定量溶液中的GAG。
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