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Author:
Date:
2018-10-20
[Abstract] Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as sulfo-NHS-LC-biotin. This protocol describes experimental procedures of sulfo-NHS-LC-biotin perfusion to evaluate retinal vascular permeability. Perfused sulfo-NHS-LC-biotin remained within vessels in wild-type postnatal day 15 (P15) retinas, confirming an intact blood-retina barrier. In contrast, sulfo-NHS-LC-biotin was occasionally detected in extravascular spaces in perfused Eogt−/− retinas suggesting a partly impaired vascular integrity in the absence of Eogt (Sawaguchi et al., 2017).
[摘要] 脑和视网膜脉管系统表现出受限的血管通透性，称为血脑屏障和血 - 视网膜屏障。 血管通透性可以通过灌注生物素的胺反应性酯衍生物如磺基-NHS-LC-生物素来评估。 该方案描述了磺基-NHS-LC-生物素灌注的实验程序，以评估视网膜血管通透性。 灌注的磺基-NHS-LC-生物素保留在野生型出生后第15天（P15）视网膜的血管内，证实了完整的血 - 视网膜屏障。 相比之下，在灌注的 Eogt - / - >视网膜中，偶尔会在血管外空间检测到磺基-NHS-LC-生物素，这表明在没有的情况下血管完整性部分受损。 Eogt >（Sawaguchi et al。>，2017）。
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