| Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
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Author:
Date:
2014-09-05
[Abstract] The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide (Asencion Diez et al., 2013). Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain (Iglesias et al., 1993). In ...
[摘要] 细胞内糖原的存在可以通过以下碘染色技术检测。 具有糖原染色的细胞为深棕色，而在其不存在时，它们保持淡黄色。 假设碘原子适合由α-聚葡萄糖形成的螺旋线圈以形成有色的糖原 - 碘络合物。 在这里，我们研究了控制该多糖的生物合成的变异链球菌（（变异链球菌））基因的表达（Asencion Diez等人 ，2013）。 因此，我们表达编码大肠杆菌中的ADP-Glc焦磷酸化酶亚基的 glgC 和 glgD 基因（ >）AC70R1-504细胞以补充该菌株的糖原的不足积累（Iglesias等人，1993）。 在对照细胞或表达无活性蛋白质的那些细胞中，通过碘染色技术检测不到多糖的合成。
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| Staphylococcus aureus Killing Assay of Caenorhabditis elegans
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Author:
Date:
2013-10-05
[Abstract] The Gram-positive bacterium Staphylococcus aureus is a human pathogen that displays virulence towards the nematode Caenorhabditis elegans. This property can be used to discover genes that are important for virulence in humans, because S. aureus possesses common virulence factors that are used in C. elegans and in humans to cause disease. S. aureus colonizes the C. elegans intestine, establishes an infection, and causes pathogenesis of the intestinal epithelium that ultimately kills the infected animal after 3 to 4 days (Sifri et al., 2003; Irazoqui et al., 2008; Irazoqui et al., 2010). The protocol described here is used to establish the rate of S. aureus-induced C. elegans death, which ...
[摘要] 革兰氏阳性菌金黄色葡萄球菌是一种人类病原体，对线虫秀丽隐杆线虫显示毒力。 这种性质可用于发现对人类毒力重要的基因，因为金黄色葡萄球菌具有在线虫和人类中引起疾病的常见的毒力因子。 金黄色葡萄球菌定殖线虫，建立感染，并引起肠上皮的发病机制，最终在3至4天后杀死感染的动物（Sifri等，2003; Irazoqui et al。，2008; Irazoqui et al ，，2010）。 这里描述的方案用于确定金黄色葡萄球菌诱导的秀丽隐杆线虫死亡率，其允许野生型和突变株的比较，从而最终有助于鉴定金黄色葡萄球菌毒力或C 线虫主机防御。 该测定法也可用于抗菌药物发现。
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