Dextran, Alexa Fluor^TM 488; 10,000 MW, Anionic, Fixable

Company: Thermo Fisher Scientific

Catalog#: D22910

Assessment of Chemosensory Response to Volatile Compounds in Healthy, Aged, and Neurodegenerative Caenorhabditis elegans Models

Cira Crespo and Roberto Grau

Published online: 2023-05-05

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In vivo Use of Dextran-based Anterograde Cortical Tracers to Assess the Integrity of the Cortical Spinal Tract

Author:

Anna M. Wiersma and Ian R. Winship,

Date:

2018-05-20

[Abstract] When injected into the motor cortex of rats, anterograde tracers label fibers of the associated descending corticospinal tract (CST) that originate from pyramidal neurons in the tracer-injected cortex. These fibers can be assessed at the level of the spinal cord to determine the integrity of the descending CST and the spatial distribution of axons in the spinal grey matter. Here we provide detailed methods on the minimally invasive stereotaxic injection of anterograde tracers into the forelimb sensorimotor representation in the rat cortex. In addition, we detail the fixing and processing of spinal tissue for assessment of CST integrity and branching into spinal grey matter. [摘要] 当注射到大鼠运动皮层时，顺行示踪剂标记伴随着皮质脊髓束下行（CST）的起源于示踪剂注射皮层中的锥体神经元的纤维。这些纤维可以在脊髓水平进行评估以确定下降的CST的完整性和脊髓灰质中轴突的空间分布。在这里，我们提供了在大鼠皮层前肢感觉运动表征的微创立体定向注射顺行示踪剂的详细方法。此外，我们详细介绍了脊柱组织的固定和加工，以评估CST的完整性和分支到脊髓灰质。

【背景】运动皮层含有上运动神经元，其产生形成CST的下行轴突并终止于脊髓。注入大鼠运动皮层的顺行示踪剂标记这些CST的降纤维，允许其在脊髓水平进行评估（图1）。在啮齿动物模型中，CST的顺行标记广泛用于评估一系列脊髓损伤模型（包括损伤）中的CST完整性（Thallmair等人，1998; Vavrek等人， 2006）和挫伤（Hill等人，2001）。此外，顺行皮质示踪剂已被用于评估不同模型的脑损伤例如皮质卒中中的遗留纤维和CST的可塑性（Wahl等人，2014; Wiersma等人， ...

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Dextran, Alexa FluorTM 488; 10,000 MW, Anionic, Fixable

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Dextran, Alexa Fluor^TM 488; 10,000 MW, Anionic, Fixable