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Author:
Date:
2016-11-20
[Abstract] The choline-containing phospholipid, phosphatidylcholine (PtdCho) is the most common mammalian phospholipid found in cell membrane (Ide et al., 2013). It is also a component of intracellular signalling pathways (Cui and Houweling, 2002). Herein is described a measure of the rate of accumulation of choline by lipid soluble PtdCho and lyso-Ptdcho which can further be discriminated by chromatographic analysis (Smith and Phyu, 2016). Determination of the accumulation of [3H-methyl]-choline into water-soluble components is also described. The procedure could be used to measure the effect of drugs and other factors on choline incorporation into phospholipids. After exposure of cells to test conditions (e.g., drugs) adherent cells in tissue culture flasks are ...
[摘要] 含胆碱的磷脂，磷脂酰胆碱（PtdCho）是在细胞膜中发现的最常见的哺乳动物磷脂（Ide等人，2013）。它也是细胞内信号传导途径的组成部分（Cui和Houweling，2002）。本文描述了脂质可溶性PtdCho和溶血磷脂酰胆碱积累胆碱的速率的量度，其可通过色谱分析进一步区分（Smith和Phyu，2016）。还描述了[3 H] - 甲基] - 胆碱在水溶性组分中的积累的测定。该程序可用于测量药物和其它因素对胆碱掺入磷脂的影响。在将细胞暴露于测试条件（例如，药物）后，将组织培养瓶中的贴壁细胞与放射性标记的[3 H] - 甲基] - 胆碱在培养基中温育15分钟（脉冲）。然后快速除去[3 H] - 甲基] - 胆碱，并在非放射性培养基（Chase）存在下继续孵育。然后通过细胞分级和测量脂质和非脂质细胞组分中的放射性来测定[3 H] - 甲基的细胞分布。
[背景] 磷脂代谢在细胞膜形成中是必需的（Ide等人，2013）和细胞信号传导（Cui和Houweling， 2002）。包含胆碱的代谢物的形成和胆碱在脂质中的积累是细胞增殖过程中的关键过程。磷脂代谢紊乱与癌症和其他疾病有关（Gibellini和Smith，2010）。这些过程的测量对于理解医学成像模态至关重要，所述医学成像模式使用[11 C] - ...
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