| Detection and Quantification of African Swine Fever Virus in MA-104 Cells
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Author:
Date:
2021-03-20
[Abstract] Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey (Cercopithecus aethiops) kidney epithelial cells, supports the detection of ASFV from field samples with a sensitivity comparable to that of primary swine macrophages. Collection of swine blood or lungs is time costing, which is often not readily available in most veterinary diagnostic laboratories. MA-104 cells could thus be used as substitute for primary swine macrophages to save significant lead time by avoiding the production of primary swine macrophages. ...
[摘要] [摘要]活的非洲猪瘟病毒（ASFV）的检测在历史上一直依赖于原代猪巨噬细胞（PSM）的使用。PSM不能复制，必须从供体猪中新鲜分离。我们先前发现，MA-104细胞（ATCC＃CRL-2378.1）是一种从非洲绿猴（Cercopithecus aethiops ）肾上皮细胞分离的商业细胞系，支持从野外样品中检测ASFV，其灵敏度可与ASFV媲美。原发性猪巨噬细胞。Ç的猪的血液或肺ollection是时间成本计算，这往往是在大多数兽医诊断实验室容易获得的。MA-104细胞因此可以用作原代猪巨噬细胞的替代品，通过避免原代猪巨噬细胞的产生来节省大量的准备时间。
[背景]非洲猪瘟病毒（ASFV）的成员，非洲猪瘟病毒科的家庭，导致野猪和家猪具有高度传染性和致命性出血热，即非洲猪瘟（ASF）。成熟的病毒颗粒（病毒粒子）直径为175-215 nm，脂质双层包裹了二十面体衣壳和180-190千碱基对的双链DNA基因组。根据宿主特征和病毒株，该病毒会引起多种症状，包括高度致死性至亚临床性（Tulman等，2009 ...
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| Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures
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Author:
Date:
2016-04-20
[Abstract] Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or polarization status of microglia, we may control their phagocytosis efficacy and therefore the cleanup process for the dead brain tissue. We cultured rat cortical neurons and microglia from the same litter of embryos. The cultured neurons are subjected to irradiation for inducing neuronal apoptosis. After labeling with propidium iodide (PI), the dead neurons (DNs) are exposed to the cultured microglia for phagocytosis assay. By counting the number of DNs in each microglia, we calculate the phagocytosis index to quantify the phagocytosis efficacy ...
[摘要] 通过吞噬作用来清除包括死亡神经元在内的死亡脑组织是脑中小胶质细胞的内源性功能，这对缺血性卒中或头部创伤后的炎症分解至关重要。通过调节小胶质细胞的功能或极化状态，我们可以控制其吞噬功效，从而控制死脑组织的清除过程。我们从相同的胚胎培养大鼠皮质神经元和小胶质细胞。培养的神经元经受辐射诱导神经细胞凋亡。用碘化丙啶（PI）标记后，将死亡神经元（DN）暴露于培养的小神经胶质细胞进行吞噬试验。通过计算每个小胶质细胞中的DN数量，我们计算吞噬指数，以量化小胶质细胞对DN的吞噬功效。方案分为4个部分：A）从产前大鼠胚胎培养大鼠皮质神经元，B）将死亡神经元作为吞噬作用靶标，C）培养大鼠脑小胶质细胞，D）定量小神经胶质细胞向死亡神经元的吞噬指数。
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