| Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
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Author:
Date:
2017-05-20
[Abstract] Plants use nitrate, nitrite, and ammonium as inorganic nitrogen (N) sources. These N compounds are included in plant tissues at various concentrations depending on the balance between their uptake and assimilation. Thus, the contents of nitrate, nitrite, and ammonium are physiological indicators of plant N economy. Here, we describe a protocol for measurement of these inorganic N species in A. thaliana shoots or roots.
[摘要] 植物使用硝酸盐，亚硝酸盐和铵作为无机氮（N）源。这些N化合物根据其吸收和同化之间的平衡以各种浓度包含在植物组织中。因此，硝酸盐，亚硝酸盐和铵的含量是植物N经济的生理指标。在这里，我们描述了在A中测量这些无机N物质的方案。 thaliana 芽或根。
背景 无机氮含量的测定对于预测植物吸收和吸收氮的能力很重要。研究人员经常使用需要昂贵设备的技术，如高效液相色谱（HPLC），用于这些测量。本协议基于通用光谱法与廉价的试剂，使其适用于许多研究人员。在酸性条件下，硝酸盐引起水杨酸的硝化，随后加入碱性溶液会导致黄色复合物。在酸性条件下，亚硝酸盐与磺胺类反应产生重氮化合物，其与N - （1-萘基）乙二胺进行重氮偶联，形成粉红色偶氮化合物。在硝普钠盐的催化作用下，铵可以测定为蓝色靛酚衍生物。每个实验程序容易，快速，简单。
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| Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
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Author:
Date:
2014-09-05
[Abstract] Galactofuranose (Galf) is a component of several polysaccharides and glycoconjugates in certain species of filamentous fungi. Galf residues are frequently found in Aspergillus glycoproteins, including N-glycans and O-mannose glycans that modify many cell wall proteins and extracellular enzymes. It is known that furanoses, contained in oligosaccharides, are detected as pyranoses after hydrolysis, and that D-galactopyranose is not contained in the galactomannoproteins of Aspergillus spp. To determine the levels of D-galactofuranose in galactomannoproteins extracted from Aspergillus nidulans (A. nidulans), we measured the amount of D-galactopyranose production after galactomannoproteins hydrolysis. The method described ...
[摘要] 半乳糖呋喃糖（Galactofuranose）（Gal ff）是某些种类的丝状真菌中几种多糖和糖缀合物的组分。 Gal< em>残基经常在曲霉属糖蛋白中发现，包括修饰许多蛋白质的聚糖和 细胞壁蛋白和细胞外酶。 已知包含在寡糖中的呋喃糖在水解后被检测为吡喃糖，并且D-吡喃半乳糖不包含在曲霉属的半乳糖蛋白中。 为了测定从构巢曲霉（构巢曲霉）提取的半乳糖蛋白中的D-半乳糖呋喃糖的水平，我们测量了半乳糖蛋白水解后D-吡喃半乳糖产生的量。 该手稿中描述的方法允许确定曲霉属中半乳糖甘露糖蛋白的D-半乳糖呋喃糖含量。
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