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Author:
Date:
2014-09-05
[Abstract] Galactofuranose (Galf) is a component of several polysaccharides and glycoconjugates in certain species of filamentous fungi. Galf residues are frequently found in Aspergillus glycoproteins, including N-glycans and O-mannose glycans that modify many cell wall proteins and extracellular enzymes. It is known that furanoses, contained in oligosaccharides, are detected as pyranoses after hydrolysis, and that D-galactopyranose is not contained in the galactomannoproteins of Aspergillus spp. To determine the levels of D-galactofuranose in galactomannoproteins extracted from Aspergillus nidulans (A. nidulans), we measured the amount of D-galactopyranose production after galactomannoproteins hydrolysis. The method described ...
[摘要] 半乳糖呋喃糖（Galactofuranose）（Gal ff）是某些种类的丝状真菌中几种多糖和糖缀合物的组分。 Gal< em>残基经常在曲霉属糖蛋白中发现，包括修饰许多蛋白质的聚糖和 细胞壁蛋白和细胞外酶。 已知包含在寡糖中的呋喃糖在水解后被检测为吡喃糖，并且D-吡喃半乳糖不包含在曲霉属的半乳糖蛋白中。 为了测定从构巢曲霉（构巢曲霉）提取的半乳糖蛋白中的D-半乳糖呋喃糖的水平，我们测量了半乳糖蛋白水解后D-吡喃半乳糖产生的量。 该手稿中描述的方法允许确定曲霉属中半乳糖甘露糖蛋白的D-半乳糖呋喃糖含量。
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