|
Author:
Date:
2013-04-20
[Abstract] Rosetting, i.e. the capacity of Plasmodium falciparum-infected red blood cells (iRBCs) to bind two or more uninfected red blood cells (RBCs) is associated with severe malaria in African children. Disruption of rosettes using small soluble inhibitors or specific antibodies is viewed as an interesting strategy to treat or prevent severe malaria manifestations. The protocol presented here describes an assay to monitor rosette dissociation, validated for the Palo Alto VarO, IT4/R29 and 3D7/PF13 rosetting clones (Vigan-Womas et al., 2011).
[摘要] Rosetting，即恶性疟原虫感染的红细胞（iRBC）结合两种或更多未感染的红细胞（RBC）的能力与非洲儿童中的严重疟疾有关 。 使用小的可溶性抑制剂或特异性抗体破坏玫瑰花结被视为治疗或预防严重疟疾表现的有趣策略。 本文提供的方案描述了监测玫瑰花结解离的测定，验证用于Palo Alto VarO，IT4/R29和3D7/PF13玫瑰花结的克隆（Vigan-Womas等人，2011）。
|