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Author:
Date:
2015-05-05
[Abstract] We made the method for Arabidopsis metabolome analysis based on direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS) (IonSpec). This method was sufficiently applied to metabolic phenotyping of Arabidopsis. This method is simple in that after homogenizing samples, powdered samples are dissolved in extraction solvents (acetone and methanol) to 20% fresh weight/volume. Extracted sample solutions are dried and dissolved in 50% (v/v) acetonitrile. Mass analysis using FT-ICR/MS (IonSpec) is performed in positive and negative ionization operation modes. Mass spectra are acquired over the 100-1,000 m/z range and accumulated to improve the S/N ratio.
[摘要] 我们基于直接输注傅立叶变换离子回旋共振质谱（FT-ICR/MS）（IonSpec）制备了拟南芥分析的方法。 该方法充分应用于拟南芥的代谢表型。 该方法的简单之处在于，在均质化样品后，将粉末样品溶解在提取溶剂（丙酮和甲醇）中至20％鲜重/体积。 将提取的样品溶液干燥并溶解在50％（v/v）乙腈中。 使用FT-ICR/MS（IonSpec）的质量分析在正和负电离操作模式中进行。 质谱在100-1,000m/z范围内获得并累积以提高S/N比。
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