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RNase AWAYTM Surface Decontaminant

RNase AWAY TM表面净化剂

Company: Thermo Fisher Scientific
Catalog#: 7005-11
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Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue
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2015-09-20
[Abstract]  This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incognita) in Arabidopsis roots using DIG (Digoxigenin-11dUTP) labeling coupled with AP (alkaline phosphatase) and nitro-blue tetrazolium/5-bromo-4-chloro-3'-indolylphosphate-based detection. This method is applicable to any other Arabidopsis root tissue. [摘要]  该方案描述了原位 RT-PCR的有效方法,其被开发用于直接在由胞囊线虫Heterodera schachtii诱导的线虫摄食位点的薄横截面中定位特异性基因表达。 在拟南芥根中( H。schachtii )或根结线虫 Meloidogyne incognita 使用与AP(碱性磷酸酶)和硝基蓝四唑/5-溴-4-氯-3'-吲哚磷酸盐基检测偶联的DIG(地高辛-11dUTP)标记。 该方法适用于任何其他拟南芥根组织。

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