| Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons
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Author:
Date:
2014-07-05
[Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in vivo model for the identification of multiple axon guidance molecules and mechanisms. These axons extend ventrally from the dorsal spinal cord and cross the midline at the floor plate, before making a sharp rostral turn towards the head. This simple trajectory has facilitated the identification of many axon guidance molecules, because perturbation of the stereotypical guidance decisions as a result of genetic manipulations can be easily identified. The open-book assay is a method to ...
[摘要] 成功的神经电路形成依赖于在发展期间轴突朝向其靶标的精确导航。轴突由短程和长程，吸引和排斥线索的组合引导。发育中的脊髓的连合轴突提供了用于多轴突导向分子和机制的鉴定的信息的体内模型。这些轴突从背侧脊髓向腹侧延伸并在底板处穿过中线，然后朝向头部进行尖锐的嘴唇转向。这个简单的轨迹有助于许多轴突指导分子的识别，因为可以容易地识别作为遗传操作的结果的定型指导决定的干扰。开放书测定是评估脊柱连合轴突的轨迹的方法。脊髓被解剖出来，在屋顶板上打开并固定平。亲脂性荧光染料DiI的点状注射用于在显微镜和分析之前追踪连合轴突轨迹。
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| Transpharyngeal Exposure of GnRH Neurons
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Author:
Date:
2014-03-20
[Abstract] The neurons secreting gonadotropin-releasing hormone (GnRH) control fertility in all mammalian species. However, investigations of this neuronal population are difficult as the cell bodies of the approximately 800 GnRH neurons are scattered through the basal forebrain ranging from the olfactory bulbs through to the base of the hypothalamus. While acute brain slice preparations enable the electrophysiological characteristics of these cells to be determined in vitro, their topography has made investigations in vivo extremely difficult. We detail here a surgical approach that allows GnRH neurons at the base of the hypothalamus to be assessed in vivo in the anesthetized mouse (Figure 1). This procedure enables electrical recordings to be made from neurons located on ...
[摘要] 分泌促性腺激素释放激素（GnRH）的神经元控制所有哺乳动物物种的生育力。 然而，对于这种神经元群体的研究是困难的，因为大约800个GnRH神经元的细胞体通过从嗅球到下丘脑的基底的基底前脑分散。 虽然急性脑切片制剂使得这些细胞的电生理特征在体外得以确定，但它们的形态使得体内的研究非常困难。 我们在这里详细介绍一种手术方法，允许在麻醉小鼠体内评估下丘脑基部的GnRH神经元（图1）。 该过程使得能够从位于小鼠脑的腹侧表面上的神经元进行电记录（Constantin等人，2013）。
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