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ALT (GPT) detection kit

ALT（GPT）检测试剂盒
Company: Roche Diagnostics
Catalog#: 11876805216
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Blood AST, ALT and UREA/BUN Level Analysis
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Date:
2013-10-05
[Abstract]  AST (aspartate aminotransferase; GOT, glutamate oxalacetate transaminase) and ALT (alanine aminotransferase; GPT, glutamate pyruvate transaminase) are sensitive indicators to monitor the liver function under drugs treatment or with acute viral hepatitis. The elevated AST and ALT values in the blood sample indicate liver damage or injury. The determination of urea is the most widely used for the evaluation of kidney function. This protocol is for the quantitative determination of AST, ALT and UREA/BUN in serum and plasma on Roche automated clinical chemistry analyzers. The principle is shown below:

For AST:
α-ketoglutarate + L-aspartate  ... [摘要]  AST（天冬氨酸氨基转移酶; GOT，谷氨酸草乙酸转氨酶）和ALT（丙氨酸氨基转移酶; GPT，谷氨酸丙酮酸转氨酶）是监测药物治疗或急性病毒性肝炎的肝功能的敏感指标。血液样品中升高的AST和ALT值指示肝损伤或损伤。尿素的测定是最广泛地用于评价肾功能的。该方案用于在Roche自动化临床化学分析仪上定量测定血清和血浆中的AST，ALT和UREA/BUN。原则如下所示：

对于AST：
alpha-ketoglutarate + L-天冬氨酸  L-苹果酸+ NAD +苹果酸脱氢酶催化这种平衡反应）
光度测定的NADH降低的速率与草酰乙酸盐的形成速率成正比，因此与AST活性成正比。上述反应在37℃下进行，在340nm的波长下测量。

对于ALT： ...

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