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Author:
Date:
2015-07-20
[Abstract] The pathogenic fungus Ophiostoma novo-ulmi spreads within the secondary xylem vessels of infected elm trees, causing the formation of vessel plugs due to tyloses and gels, which ultimately result in Dutch elm disease. Foliage discoloration, wilting and falling from the tree are typical external leaf symptoms of the disease followed by the subsequent death of sensitive trees. Cellulolytic enzymes produced by the fungus are responsible for the degradation of medium molecular weight macromolecules of cellulose, resulting in the occurrence of secondary cell wall ruptures and cracks in the vessels but rarely in the fibers (Ďurkovič et al., 2014). The goal of this procedure is to evaluate the extent of cellulose degradation by a highly aggressive strain of O. novo-ulmi ...
[摘要] 病原真菌新孢子虫在受感染的榆树的次生木质部导管内扩散，导致由于菌丝和凝胶而形成血管栓塞，最终导致荷兰榆树病。叶子变色，枯萎和从树上掉下是典型的外部叶症状的疾病，随后是敏感树的死亡。由真菌产生的纤维素分解酶负责纤维素的中等分子量大分子的降解，导致在血管中发生继发性细胞壁破裂和裂纹，但是在纤维中很少发生破裂（Jurkovic等人， ，2014）。该程序的目的是通过高侵蚀性的O应变评价纤维素降解的程度。 novo-ulmi ssp。 美国× novo-ulmi 。尺寸排阻色谱法（SEC）比较了感染的和未感染的榆树之间的纤维素的分子量分布，并揭示了纤维素的大分子性状的变化，包括分子量，聚合度和多分散指数。 13 C魔幻角旋转核磁共振（ 13 C MAS NMR）光谱有助于鉴定和定量由于降解而导致的结晶和非结晶纤维素区域的损失。本文所述的方法也可以容易地用于感染各种纤维素降解真菌的其它木本植物。
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