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Author:
Date:
2013-08-20
[Abstract] Neurons consist of four elements, the soma, dendrite, axon and terminal. They work in concert as the input (soma and dendrite) and output (axon and terminal) parts of neuronal transmission. To function and maintain neuronal activity and metabolisms, proteins and organelles should be transported from soma to terminal via anterograde axonal transport, and also from terminal to soma via retrograde transport. By utilizing these transport systems, neural projection is traced by injecting tracers into local sites of interest. Furthermore, neurochemical properties, such as glutamatergic and GABAergic, can be determined by combining retrograde and anterograde tracing with fluorescent in situ hybridization and immunofluorescence.
[摘要] 神经元由四个元素组成，即索马，树突，轴突和末端。 它们作为神经元传播的输入（神经和树突）和输出（轴突和末端）部分协同工作。 为了起到和维持神经元活动和代谢的作用，蛋白质和细胞器应通过逆行轴突运输从脊髓运输到终末，也可以通过逆行运输从末端转移到血管。 通过利用这些运输系统，通过将示踪剂注入到感兴趣的地方来跟踪神经投影。 此外，神经化学性质，如谷氨酸能和GABA能，可以通过逆行和顺行跟踪与荧光原位杂交和免疫荧光结合来确定。
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