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Author:
Date:
2013-05-20
[Abstract] The Infinium Human Methylation 450 BeadChip technology allows the rapid quantitative DNA methylation analysis of more than 485,000 CpG dinucleotides located across the genome. The method utilizes sodium bisulfite treatment of genomic DNA to convert unmethylated cytosine residues into uracils whereas methylated cytosines remain unchanged. Modified DNA is then whole genome amplified, fragmented and hybridized to locus-specific oligomer probes linked to individual beads on a BeadChip. Hybridization is followed by single-base extension of the oligomer with a labeled nucleotide. The BeadChip is subsequently fluorescently stained and scanned to measure the intensities of the beads corresponding to the unmethylated and methylated CpG sites.
[摘要] Infinium人甲基化450 BeadChip技术允许对跨越基因组的超过485,000个CpG二核苷酸进行快速定量DNA甲基化分析。 该方法利用亚硫酸氢钠处理基因组DNA以将未甲基化的胞嘧啶残基转化为尿嘧啶，而甲基化的胞嘧啶保持不变。 然后将修饰的DNA全基因组扩增，片段化并与连接到BeadChip上的单个珠的基因座特异性寡核苷酸探针杂交。 杂交之后是具有标记的核苷酸的寡聚物的单碱基延伸。 随后对珠粒芯片进行荧光染色和扫描，以测量对应于未甲基化和甲基化的CpG位点的珠子的强度。
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