Author:
Date:
2012-12-05
[Abstract] Grafting is a very useful technique for testing the transmission of long-distance signals in plants and is used in agriculture and gardening for different purposes. This protocol, based on a previously published one (Jackson et al., 1998), describes a grafting method for potato plants, which has successfully been used to test the transmission of tuber-inducing signals (Martin et al., 2009; González-Schain et al., 2012). We describe first the procedures for plant growth and then the grafting procedure. Although this method has been used for grafting plants that were initially grown in vitro, it should work as well with plants grown from tubers in soil. This protocol probably works well for other plant species, with small modifications.
[摘要] 接枝是一种非常有用的技术,用于测试植物中长距离信号的传输,并用于农业和园艺用于不同的目的。 该方案基于先前公开的方案(Jackson等人,1998)描述了马铃薯植物的接枝方法,其已经成功地用于测试块茎诱导信号的传播(Martin等人, et al。,2009;González-Schain et al。,2012)。 我们首先描述植物生长的程序,然后描述嫁接程序。 虽然这种方法已经用于移植最初在体外生长的植物,但是它应当与从土壤中的块茎生长的植物一起使用。 这个协议可能适用于其他植物物种,有小的修改。
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