|
Author:
Date:
2014-03-05
[Abstract] Transposable elements represent a major part of any eukaryotic genomes. Notably in plants they can account for more than 80% of the whole genomic sequence (such as in maize). Due to their mobility across the genome, they can act as mutagens but can also be considered as an important source of genetic diversity. It has been shown that they may be activated following various stresses, and it has been assumed that they may contribute to genome evolution and adaptation. Molecular methods have thus been proposed to allow identification of new transposition events, or more generally to tag transposable element insertion site polymorphisms. Sequence-Specific Amplification Polymorphism (SSAP) is a high throughput method derived from AFLP, which has been first tested on the barley genome (Waugh et ...
[摘要] 转座元件代表任何真核基因组的主要部分。值得注意的是，在植物中，它们可以占全部基因组序列的80％以上（例如在玉米中）。由于它们在基因组上的移动性，它们可以作为诱变剂，但也可以被认为是遗传多样性的重要来源。已经表明，它们可以在各种胁迫之后被激活，并且已经假定它们可以有助于基因组进化和适应。因此已经提出分子方法以允许鉴定新的转座事件，或更通常地标记可转座元件插入位点多态性。序列特异性扩增多态性（SSAP）是源自AFLP的高通量方法，其已经在大麦基因组上首先测试（Waugh等人，1997）。与AFLP相比，其标记TE的效率基于锚定在感兴趣的TE序列中的特异性引物的使用，需要先前表征所调查的TE。 SSAP因此可以用于鉴定TE插入位点附近的任何基因组重组，并且仍然是分析TE的进化动力学的有效方法。
|