Pentylenetetrazole

Company: Sigma-Aldrich

Catalog#: P6500

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Pentylenetetrazole (PTZ)-induced Convulsion Assay to Determine GABAergic Defects in Caenorhabditis elegans

Author:

Shruti Thapliyal and Kavita Babu,

Date:

2018-09-05

[Abstract] Pentylenetetrazole (PTZ) is a GABA_A receptor antagonist and is used to monitor presynaptic defects in the release of the inhibitory neurotransmitter GABA. PTZ is a competitive inhibitor of GABA, and prevents binding of GABA on the GABA_A receptors present on the surface of muscle. In the absence of GABA binding, the excitatory to inhibitory signal ratio increases resulting in a convulsive phenotype. This assay provides a fast and reliable method to detect presynaptic defects in GABAergic synaptic transmission. The assay is based on correlating the extent of convulsions with the degree of presynaptic GABA release defects. [摘要] 戊四唑（PTZ）是GABA A 受体拮抗剂，用于监测抑制性神经递质GABA释放中的突触前缺陷。 PTZ是GABA的竞争性抑制剂，并且阻止GABA与肌肉表面上存在的GABA A 受体结合。在没有GABA结合的情况下，抑制信号比的兴奋性增加导致惊厥表型。该试验提供了一种快速可靠的方法来检测GABA能突触传递中的突触前缺陷。该测定基于将惊厥的程度与突触前GABA释放缺陷的程度相关联。

【背景】突触是介导突触传递的不对称细胞间连接。它们构成了能够执行复杂行为的大脑电路的基本单元。动作电位的到达通过电压门控钙通道引起钙流入，导致质膜的去极化。在这种突触前去极化后，突触囊泡与质膜融合，通过高度控制的过程将其内容物释放到突触间隙中（Sudhof，1995; Sudhof，2004）。

运动是 Caenorhabditis elegans 中突出的行为输出。产生协调的背腹窦弯曲的神经回路允许 C中的正常运动。线虫。运动行为在多个层面上进行协调，并且涉及由中间神经元处理的多个感觉神经元整合多种感觉线索，并最终在神经肌肉接头（NMJ）处直接变化（de Bono和Maricq，2005; Bargmann，2012）。 C.线虫 NMJ有三个主要组成部分;兴奋性胆碱能运动神经元，抑制性GABA能神经运动神经元和突触后肌细胞（White et ...

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