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Author:
Date:
2015-06-20
[Abstract] The intratracheal instillation technique is used to deliver a variety of agents to the lungs ranging from pathogens (bacteria, viruses), toxins, to therapeutic agents. To model lung inflammation and injury, LPS can be administrated via intranasal, intratracheal, or aerosol approaches. Each technique has its limitations. The intratracheal technique can involve the non-invasive instillation method (via the oro-tracheal route) or a direct injection into the trachea. Here, we describe an optimized method for direct visual instillation of LPS via the non-invasive oro-tracheal route.
[摘要] 气管内滴注技术用于将各种试剂递送到肺，范围从病原体（细菌，病毒），毒素，到治疗剂。 为了模拟肺炎症和损伤，LPS可以通过鼻内，气管内或气雾剂方法施用。 每种技术都有其局限性。 气管内技术可涉及非侵入性滴注方法（通过经气管途径）或直接注射到气管中。 在这里，我们描述了通过非侵入性气管途径直接视觉滴注LPS的优化方法。
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