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Author:
Date:
2014-11-20
[Abstract] This protocol details the isolation of enriched Golgi membranes from rat liver, using discontinuous density gradient centrifugation. This high-yield extraction method is useful for several applications, including immunoprecipitation of solubilised Golgi membrane proteins (preparation included) and electron microscopy. Protocol adapted from Leelavathi et al. (1970).
[摘要] 该协议详述了使用不连续密度梯度离心从大鼠肝脏分离富集的高尔基体膜。 这种高产率提取方法可用于几种应用，包括溶解的高尔基膜蛋白（包括制备）和电子显微镜的免疫沉淀。 从Leelavathi等人改编的方案（1970）。
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