| Induction of Tigecycline Resistance in Acinetobacter baumannii
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Author:
Date:
2014-12-05
[Abstract] Multidrug resistance Acinetobacter baumannii (A. baumannii) (MDRAB) has emerged as a serious threat in hospitals in recent years. Currently, there are few antibiotics, including tigecycline, available to treat infections caused by MDRAB effectively. Both tigecycline-resistant and tigecycline-susceptible isogenic strains of MDRAB are valuable in understanding the mechanisms underlying tigecycline resistance. To get the isogenic strains in the laboratory, we describe a protocol for induction of tigecycline resistance in A. buamannii by serial passage to plates with tigecycline of different concentrations. The minimal inhibitory concentration of A. baumannii by tigecycline was determined according to the protocol “Minimal Inhibitory Concentration (MIC) ...
[摘要] 多药耐药近年来，在医院中，抗巨细胞鲍氏不动杆菌（HA）抗体（MDRAB）已经成为严重的威胁。 目前，几乎没有抗生素，包括替加环素，可有效治疗MDRAB引起的感染。 MDRAB的替加环素抗性和替加环素敏感的同基因菌株对于了解替加环素抗性的机制是有价值的。 为了在实验室中获得同基因菌株，我们描述了在A中诱导替加环素抗性的方案。 buamannii）通过连续进入具有不同浓度替加环素的平板。 A的最小抑制浓度。 根据方案"最小抑制浓度（MIC）测定法"确定替加环素的阿米巴 鲍氏不动杆菌" "（Lin等人，2014b）。
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| Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
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Author:
Date:
2014-12-05
[Abstract] Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an antimicrobial. Multidrug resistance Acinetobacter baumannii (A. baumannii) has emerged in recent years. Accurate determination of resistance by MIC assay is important in coping with this superbug. Here we described a protocol for determining MIC for A. baumannii in hope of assisting researchers and physicians in confirming resistance of clinical isolates correctly.
[摘要] 最小抑制浓度（MIC）是可以在过夜孵育后抑制微生物可见生长的抗微生物剂的最低浓度。 MIC测定不仅用作治疗临床医生的细菌感染的诊断工具，而且用作评价抗微生物剂的功效的研究方法。 近年来出现了多药耐药性鲍曼不动杆菌（Eminetobacter baumannii）（鲍曼不动杆菌）。 通过MIC测定的抗性的精确测定对于应对该超级细菌是重要的。 这里我们描述了用于确定 A的MIC的协议。 baumannii 希望协助研究人员和医生正确地确认临床分离株的耐药性。
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