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Author:
Date:
2016-09-05
[Abstract] Iron in blood plasma is bound to its transport protein transferrin, which delivers iron to most tissues. In iron overload and certain pathological conditions, the carrying capacity of transferrin can become exceeded, giving rise to non-transferrin-bound iron, which is taken up preferentially by the liver, kidney, pancreas, and heart. The measurement of tissue transferrin- and non-transferrin-bound iron (TBI and NTBI, respectively) uptake in vivo can be achieved via intravenous administration of 59Fe-labeled TBI or NTBI followed by gamma counting of various organs. Here we describe a detailed protocol for the measurement of TBI and NTBI uptake by mouse tissues.
[摘要] 血浆中的铁结合其转运蛋白转铁蛋白，其将铁递送至大多数组织。 在铁过载和某些病理状况下，转铁蛋白的携带能力可能超过，导致非转铁蛋白结合的铁，其优先被肝脏，肾脏，胰腺和心脏摄取。 分别测量组织转铁蛋白和非转铁蛋白结合的铁（分别为TBI和NTBI）在体内的摄取可以通过静脉内施用59 Fe标记的TBI或 NTBI，然后是各种器官的γ计数。 在这里我们描述了测量小鼠组织的TBI和NTBI摄取的详细协议。
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