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Author:
Date:
2016-09-20
[Abstract] During the last 20 years intestinal mesenchymal cells (IMCs) have emerged as an important cell type that plays a central role in intestinal development and homeostasis, by providing both structural support and growth regulatory elements. IMCs also actively participate in wound healing responses, thus regulating pathologic conditions such as tissue repair, inflammation, fibrosis and carcinogenesis (Powell et al., 2011). We have recently demonstrated that intestinal mesenchymal-specific signals play important in vivo physiological roles in intestinal inflammation and carcinogenesis (Koliaraki et al., 2012; Roulis et al., 2014; Koliaraki et al., 2015). Here we describe the enzymatic method used for the isolation and culture of mesenchymal cells ...
[摘要] 在过去20年间，肠间质细胞（IMC）已经作为重要的细胞类型出现，通过提供结构支持和生长调节元件在肠发育和体内平衡中起着中心作用。 IMC还积极参与伤口愈合反应，从而调节病理状况，例如组织修复，炎症，纤维化和癌发生（Powell等人，2011）。 我们最近已经证明肠间充质特异性信号在肠炎症和癌发生中在体内起重要的生理作用（Koliaraki等人，2012; Roulis等人，/em>。，2014; Koliaraki 。。，2015）。 在这里我们描述了用于从成年小鼠肠道分离和培养间充质细胞的酶法。
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