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Author:
Date:
2014-07-05
[Abstract] In Arabidopsis thaliana, hypocotyl micrografting has been used to investigate transport of flowering signals, mobile silencing signals and other peptides, proteins and secondary compounds. The effects of transported signals on target tissues require that a good vascular connection is re-established across the graft junction between the cut hypocotyls (stumps) of the root (rootstock) and shoot (scion) tissues. We outline here a method that requires only that the cut stumps be placed in close proximity, so that they touch, followed by 3-5 days of undisturbed recovery time during which the grafts are allowed to dry out somewhat. This method is quick, easy to monitor and has up to 90% success rate.
[摘要] 在拟南芥中，下胚轴微量移植已经用于研究开花信号，移动沉默信号和其他肽，蛋白质和次级化合物的转运。 运输信号对靶组织的影响需要在根（根茎）和芽（接穗）组织的切割的下胚轴（树桩）之间的移植物接合处重建良好的血管连接。 我们在这里概述了一种方法，其仅需要将切割的树桩放置在非常接近的位置，使得它们接触，随后是3-5天的不受干扰的恢复时间，在此期间允许移植物稍微干燥。 这种方法快速，易于监控，并具有高达90％的成功率
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