| In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
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Author:
Date:
2016-10-20
[Abstract] Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, which can be applied to other organs. In this protocol fluorescent-labeled tumor cells are transferred to recipient mice expressing fluorescently tagged immune cells. Tumor-immune cell interactions in the lung are then imaged by confocal or two photon microscopy. Analysis of tumor interactions with immune cells using this protocol should aid in a better understanding of the importance of these interactions and their role in developing ...
[摘要] 免疫治疗已经通过激活免疫系统抵抗癌症而显示出巨大的治疗潜力。然而，关于在肿瘤和免疫细胞之间发生的相互作用的具体动力学知之甚少。在这个协议中，我们描述了一种新的方法来可视化肿瘤和免疫细胞在活小鼠的肺中的相互作用，可以应用到其他器官。在该协议中，荧光标记的肿瘤细胞转移到表达荧光标记的免疫细胞的受体小鼠。然后通过共聚焦或双光子显微镜对肺中的肿瘤 - 免疫细胞相互作用成像。使用该方案分析肿瘤与免疫细胞的相互作用应当有助于更好地理解这些相互作用的重要性及其在开发免疫疗法中的作用。
[背景] ...
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| Quantification of Tumor Material Uptake
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Author:
Date:
2016-10-20
[Abstract] Extracellular tumor material including exosomes, microvesicles and apoptotic tumor debris may help cancers invade new organs. Enhancing the removal of extracellular tumor material by immune cells represents a novel immunotherapy approach for preventing cancer metastasis. This protocol quantifies the uptake and removal of extracellular tumor material from circulation and tissues by immune cells. In this assay fluorescent tumor cells are transferred into mice, and then immune cells are quantified by either flow cytometry or imaging cytometry for their uptake of tumor material.
[摘要] 包括外来体，微泡和凋亡性肿瘤碎片的细胞外肿瘤材料可以帮助癌症侵入新器官。增强免疫细胞对细胞外肿瘤材料的去除代表了用于预防癌症转移的新型免疫治疗方法。该方案定量免疫细胞从循环和组织吸收和去除细胞外肿瘤物质。在该测定中，将荧光肿瘤细胞转移到小鼠中，然后通过流式细胞术或成像细胞计量术来定量免疫细胞对肿瘤材料的摄取。
[背景] 研究已经证明，包括从肿瘤脱落的外来体，微泡和凋亡性肿瘤碎片的细胞外肿瘤材料是肿瘤转移，生长和逃避免疫应答的重要介质（Vader等人，2014; Pucci和Pittet ，2013; Pucci等人，2016）。免疫细胞具有去除，应答和转运这种循环肿瘤材料的能力（Hanna等人，2015; Pucci等人，2016; Headley等人。，2016）。该协议提供了一种新的方法来量化特定免疫细胞群体的肿瘤材料摄取，并且可以适于测试调节肿瘤材料摄取的免疫靶标。该协议可以帮助更好地理解对细胞外肿瘤材料的免疫应答，希望最终开发针对癌症发展中的细胞外肿瘤材料的新疗法。
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