{{'Search' | translate}}
 

BD IntramedicTM Polyethylene Tubing (Non-Sterile) (PE 90)

适配器和管

Company: BD
Catalog#: 427420
Bio-protocol()
Company-protocol()
Other protocol()

In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
Author:
Date:
2016-10-20
[Abstract]  Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, which can be applied to other organs. In this protocol fluorescent-labeled tumor cells are transferred to recipient mice expressing fluorescently tagged immune cells. Tumor-immune cell interactions in the lung are then imaged by confocal or two photon microscopy. Analysis of tumor interactions with immune cells using this protocol should aid in a better understanding of the importance of these interactions and their role in developing ... [摘要]  免疫治疗已经通过激活免疫系统抵抗癌症而显示出巨大的治疗潜力。然而,关于在肿瘤和免疫细胞之间发生的相互作用的具体动力学知之甚少。在这个协议中,我们描述了一种新的方法来可视化肿瘤和免疫细胞在活小鼠的肺中的相互作用,可以应用到其他器官。在该协议中,荧光标记的肿瘤细胞转移到表达荧光标记的免疫细胞的受体小鼠。然后通过共聚焦或双光子显微镜对肺中的肿瘤 - 免疫细胞相互作用成像。使用该方案分析肿瘤与免疫细胞的相互作用应当有助于更好地理解这些相互作用的重要性及其在开发免疫疗法中的作用。

[背景] ...

Comments