| An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo
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Author:
Date:
2017-01-20
[Abstract] Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord’s highly myelinated funiculi. The preparation of acute slices may offer an alternative and valuable strategy to collect structural and functional information in vitro from dorsal, lateral and ventral regions of spinal cord. Here, we describe a procedure for preparing acute slices from mouse spinal cord (Garré et al., 2016). This preparation should allow further understanding of how glial cells in spinal cord respond acutely to various inflammatory challenges.
[摘要] 病理状况如肌萎缩性侧索硬化，脊髓损伤和慢性疼痛的特征在于脊髓中星形胶质细胞和小胶质细胞的活化，并已在啮齿动物中建模。 在这些动物模型中的细胞水平的体内成像由于脊髓的高度髓鞘化的功能而受到限制。 急性切片的制备可能提供一种替代和有价值的策略，从脊髓的背侧，外侧和腹侧区域体外收集结构和功能信息。 在这里，我们描述了一种从小鼠脊髓制备急性切片的过程（Garréet al。，2016）。 这个准备应该进一步了解脊髓中的神经胶质细胞是如何急剧地反应各种炎症的挑战。
【背景】已经使用小鼠转基因技术来模拟影响脊髓的不同人类病态，其中许多特征为局部胶质激活，神经炎症的一个标志。利用基于激光扫描显微技术的共焦显微镜（White et al。，1987）和双光子显微镜（Denk等，1990），大大提高了对健康和疾病中胶质生物学的认识。 ）以无创的方式可视化活体动物中的细胞结构和亚细胞结构域;例如，表达遗传编码的记者或钙传感器的小鼠已被用于分别形成胶质结构（体细胞和过程）并研究钙动力学和信号传导（Davalos等人，2005; ...
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| In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
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Author:
Date:
2016-10-20
[Abstract] Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, which can be applied to other organs. In this protocol fluorescent-labeled tumor cells are transferred to recipient mice expressing fluorescently tagged immune cells. Tumor-immune cell interactions in the lung are then imaged by confocal or two photon microscopy. Analysis of tumor interactions with immune cells using this protocol should aid in a better understanding of the importance of these interactions and their role in developing ...
[摘要] 免疫治疗已经通过激活免疫系统抵抗癌症而显示出巨大的治疗潜力。然而，关于在肿瘤和免疫细胞之间发生的相互作用的具体动力学知之甚少。在这个协议中，我们描述了一种新的方法来可视化肿瘤和免疫细胞在活小鼠的肺中的相互作用，可以应用到其他器官。在该协议中，荧光标记的肿瘤细胞转移到表达荧光标记的免疫细胞的受体小鼠。然后通过共聚焦或双光子显微镜对肺中的肿瘤 - 免疫细胞相互作用成像。使用该方案分析肿瘤与免疫细胞的相互作用应当有助于更好地理解这些相互作用的重要性及其在开发免疫疗法中的作用。
[背景] ...
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