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QSonica XL-2000

QSonica XL-2000

Company: Qsonica
Catalog#: XL-2000
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Detection of Wnt5 in Media Conditioned by Mouse Embryonic Fibroblast
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2016-10-20
[Abstract]  This protocol describes the procedure of visualizing secreted Wnt5 protein in serum free media via western blotting. This procedure can also be used to visualize other secreted proteins larger than 10,000 daltons. The work presented in this paper visualizes Wnt5 secreted by mouse embryonic fibroblast (MEF), but can be adapted to other cell lines including those transiently transfected by plasmids. [摘要]  该协议描述了通过免疫印迹观察无血清培养基中分泌的Wnt5蛋白的过程。 该程序还可用于显现大于10,000道尔顿的其他分泌的蛋白质。 本文提出的工作可视化由小鼠胚胎成纤维细胞(MEF)分泌的Wnt5,但可以适应于其他细胞系,包括由质粒瞬时转染的那些。

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