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Author:
Date:
2016-10-05
[Abstract] The sucrose preference test (SPT) is a reward-based test, used as in indicator of anhedonia. Anhedonia, or the decreased ability to experience pleasure, represents one of the core symptoms of depression. Rodents are born with an interest in sweet foods or solutions. Reduced preference for sweet solution in SPT represents anhedonia, while this reduction can be reversed by treatment with antidepressants. SPT is carried out in the animal’s home cage. For the SPT, mice are presented with 2 dual bearing sipper tubes. One tube contains plain drinking water, and the second contains a sucrose solution. Water and sucrose solution intake is measured daily, and the positions of two bottles is switched daily to reduce any confound produced by a side bias. Sucrose preference is calculated as a ...
[摘要] 蔗糖偏好测试（SPT）是基于奖励的测试，用作快感缺乏的指示器。快感缺乏或经历快乐的能力降低，代表抑郁症的核心症状之一。啮齿动物出生时对甜的食物或溶液有兴趣。 SPT中对甜味溶液的降低偏好代表了快感缺乏，而这种减少可以通过用抗抑郁药治疗逆转。 SPT在动物的家笼中进行。对于SPT，给小鼠提供2个双轴承吸管。一个管含有普通饮用水，第二个管含有蔗糖溶液。每天测量水和蔗糖溶液的摄入量，并且每天切换两个瓶的位置以减少由侧偏压产生的任何混杂。蔗糖偏好计算为蔗糖摄取量相对于流体摄入总体积的百分比，并在测试期间平均。在这里，我们提出我们的协议，已经能够检测在慢性抑郁模型小鼠中的快感。
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