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Author:
Date:
2014-04-05
[Abstract] The goal of this protocol is to assay for defects in synthesis/secretion/release of seed coat mucilage by ruthenium red staining of mature whole seeds.
The mucilage secretory cells of the Arabidopsis seed coat synthesize and secrete a large quantity of primarily pectinaceous mucilage to a ring-shaped apical domain during their differentiation. This makes them an excellent model system to identify genes involved in both cell wall synthesis and secretion (et al., 2000). When wild-type seeds are incubated in ruthenium red stain, hydrated mucilage is extruded from epidermal cells and a ‘halo’ of red-stained mucilage is observed surrounding the seed (Western et al., 2000). Reduced mucilage staining may result from defects in cell wall biosynthesis, ...
[摘要] 该协议的目的是通过成熟全种子的钌红染色来测定种皮粘液合成/分泌/释放中的缺陷。拟南芥种皮的粘液分泌细胞合成 并在其分化期间将大量的主要果胶粘液分泌到环状顶端结构域。 这使得它们是鉴定参与细胞壁合成和分泌的基因的极好的模型系统（等人，2000）。 当野生型种子在钌红染色中孵育时，水合粘液从表皮细胞中挤出，并且在种子周围观察到红色染色的粘液的"晕"（Western等人，2000）。 减少粘液染色可能由细胞壁生物合成，分泌缺陷或水合作用时释放受损而导致。
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