| An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens
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Author:
Date:
2016-10-05
[Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have serious drawbacks. One alternative is the use of beneficial bacteria to improve plant health. Pseudomonas fluorescens has been shown to trigger a plant-mediated resistance response in aboveground plant tissues against fungal, oomycete, bacterial, and viral pathogens. Triggered plant resistance exploits mechanisms of the plant immune system through a priming state that provides plants with enhanced capacity for rapid and strong activation of defense responses after ...
[摘要] 葡萄（葡萄（Vitis vinifera）L.）易受一系列疾病的影响，其中包括由坏死性真菌葡萄孢菌引起的灰霉病，其降低葡萄生产力和质量。为了确保令人满意的产量和收获质量，需要许多化学杀真菌剂，但它们具有严重的缺点。一个替代方案是使用有益细菌来改善植物健康。已经显示荧光假单胞菌在地上植物组织中触发植物介导的对真菌，卵菌，细菌和病毒病原体的抗性反应。触发的植物抗性通过启动状态利用植物免疫系统的机制，其提供植物在病原体感染后快速和强烈地激活防御反应的增强能力，导致较低的适合度成本。有益细菌的引发应答包括防御相关基因的诱导表达，细胞壁增强和病原体感染后次生代谢产物的产生。在该方案中，我们描述了根据Verhagen评价有益细菌荧光假单胞菌PTA-CT2对葡萄植物的引发状态及其对灰葡萄孢的抗性水平的实验设计等。 （2011）和Gruau等人。 （2015）。
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| 15N-nitrate Uptake Activity and Root-to-shoot Transport Assay in Rice
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Author:
Date:
2016-08-20
[Abstract] 15N is a nonradioactive heavy isotope of nitrogen, widely used for biochemical and physiological research in plants. For instance, 15N-KNO3 was used as the nitrogen source in plants in order to investigate nitrate uptake activity and transport from roots to shoots (Lin et al., 2008). Here, we describe a detailed pipeline used for labeling living rice (Oryza sativa) plants with 15N-KNO3 and determination of net nitrate uptake and transport activity, and this protocol was proved to be valid in Arabidopsis and rice (Lin et al., 2008; Hu et al., 2015).
[摘要] N是氮的非放射性重同位素，广泛用于植物的生化和生理研究。 例如，使用N-KNO 3作为植物中的氮源，以研究硝酸盐吸收活性和从根到芽的转运（Lin等人 。， 2008）。 在这里，我们描述了用于标记具有 N-KNO 3的活水稻（>稻）植物和测定硝酸盐硝酸盐吸收的详细管道 和转运活性，并且该方案在拟南芥（Arabidopsis）和水稻中被证明是有效的（Lin等人，2008; Hu等人， 2015）。
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