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Author:
Date:
2016-10-05
[Abstract] The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the cells. However, during stressful conditions such as oxidative stress, ROS levels increase causing damages to different molecules like DNA, lipids and proteins. Increased levels of ROS have been associated with a growing list of different diseases. In this protocol, we used MitoSOX and CellROX Green oxidative stress probes to label the intracellular ROS and detect the fluorescence using cell sorting and confocal analyses.
[摘要] 自由基的产生是正常细胞代谢的结果。 自由基涉及无数不同的细胞和生物学功能，例如信号传导，增殖，细胞死亡，衰老，炎症等。 在生理条件下，活性氧（ROS）的水平被细胞严格调节。 然而，在应激条件例如氧化应激期间，ROS水平增加导致对不同分子如DNA，脂质和蛋白质的损伤。 增加的ROS水平与不断增加的不同疾病列表相关联。 在这个协议中，我们使用MitoSOX和CellROX绿色氧化应激探针标记细胞内ROS和使用细胞分选和共聚焦分析检测荧光。
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