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Parafilm

Company: VWR
Catalog#: 291-1213
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Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
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Date:
2018-04-20
[Abstract]  Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips). [摘要]  昆虫损伤的定量是鉴定植物抗性的重要指标。 在筛选宿主植物对蓟马的抗性时,将总的受损叶面积用作确定抗性水平的标准。 在这里我们提出了一种客观的新方法,用免费提供的软件程序Ilastik和ImageJ来分析叶盘上的蓟马损伤。 该协议的开发是为了筛选超过40个辣椒品系对西方花蓟马和西蓟马蓟马(洋葱蓟马)的抗性。

【背景】昆虫损伤的定量是鉴定植物抗性的重要指标。在筛选宿主植物对蓟马抗性的程序中,总损伤叶面积被用作确定抗性水平的标准。蓟马损害的特点是银色斑点与完整叶片区域显示出高对比度,但喂食点还包括从深绿色到棕色的深色区域。这些叶片的逐渐变色太细微,无法用诸如Winfolia等程序精确量化( http:// www .regentinstruments.com / assets / winfolia_software.html )或ImageJ(Rasband,2011)。结果,蓟马损害通常由评估样品的个体评分。将样本分为表示损害数量的类别(Mirnezhad等人,2010; Maharijaya等人,2011和2012),或损害估计为最接近的1 (Leiss等人,2009; ...

Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi and Plants to Follow Fungal Phosphate Metabolism
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Date:
2017-10-20
[Abstract]  In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the ‘Hartig net’, where exchanges between the two partners take place. Finally, the efficiency of ectomycorrhizal fungi to improve the phosphorus nutrition of their host plants will depend on the regulation of phosphate transfer from the fungal cells to plant cells in the Hartig net through as yet unknown mechanisms. In order to investigate these mechanisms, we developed an in vitro experimental device mimicking the common apoplasm of the ectomycorrhizae (the Hartig net) to study the phosphorus metabolism in the ... [摘要]  在外生菌根植物中,真菌细胞定植于其宿主植物的根部,以产生称为外生菌根的新器官。在这些新器官中,真菌细胞定居在皮质细胞的壁上,与称为“Hartig网”的空间中的植物细胞在同一质粒中沐浴,两地之间的交流发生。最后,外源菌根真菌提高其宿主植物的磷营养的效率将取决于通过尚未知的机制调节从真菌细胞到Hartig网中植物细胞的磷酸转移。为了研究这些机制,我们开发了一种体外实验装置,模拟外生菌根(Hartig网)的常见质粒来研究外生菌根真菌中的磷代谢。 当真菌细胞与寄主植物“Pinus pinaster”的植物细胞相关联时。该装置可用于监测从先前与 32 P-正磷酸盐一起培养的真菌的磷酸盐外排。
【背景】已知菌根真菌和植物之间的关联可以改善植物P营养(由Smith和Read,2008; Plassard和Dell,2010; Cairney,2011; ...

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