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Author:
Date:
2017-10-05
[Abstract] Northern corn leaf blight (NCLB) is a serious foliar disease of maize (Zea mays) worldwide and breeding for resistance is of primary importance for maize crop protection. Phenotyping for NCLB resistance is well established in the field, but such experiments depend on suitable environmental conditions and are seasonal. Here we describe a greenhouse seedling approach that is suitable for testing thousands of seedling plants in a single experiment with a duration of 37 days. Three scoring methods were used to quantify the disease severity: the area under the disease progress curve (AUDPC), the primary diseased leaf area of the inoculated leaves at 16 days post inoculation (PrimDLA at 16 dpi) and the incubation period (IP) that was determined as days from inoculation to symptom ...
[摘要] 北方玉米叶枯病（NCLB）是世界范围内严重的玉米叶片病（Zea mays），对抗玉米作物保护最为重要。 NCLB抗性的表型在该领域已经确立，但是这种实验取决于合适的环境条件并且是季节性的。在这里，我们描述了一种温室幼苗方法，适用于在单次实验中测试数千株幼苗，持续时间为37天。使用三种评分方法来定量疾病严重程度：疾病进展曲线（AUDPC）下的面积，接种后16天接种的叶的主要病叶面积（16dpi的PrimDLA）和潜伏期（IP），被确定为从接种到症状外观的天数。通过测试多样化的玉米基因型，观察到三种不同方法之间的高度相关性（81.9％至94.1％），表明每种评分方法可用于疾病定量。因此，开发的幼苗测定作为在温室条件下表型NCLB抗病性的相对简单且高通量的方法。
【背景】北方玉米叶枯病（NCLB）是全球威胁到玉米生产的无处不在的叶枯病（Welz和Geiger，2000）。该疾病是由有利于高湿度和凉爽的温度环境的半抗生物真菌（Exserohilum turcicum）（（Setosphaeria turcica ...
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