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Author:
Date:
2016-09-20
[Abstract] Confocal laser scanning microscopy in combination with fluorescent proteins is a powerful tool for the study of sexual reproduction and other developmental processes in plants. In order to understand the origin and localization of fluorescent signals in a complex tissue, staining of cell outlines is often mandatory. Cell wall staining with SCRI Renaissance 2200 (SR2200) has recently been described as a method of choice to study plant reproductive processes (Musielak et al., 2015). In this protocol, we present detailed instructions on the use of SR2200 to stain cell walls in different Arabidopsis tissues.
[摘要] 共聚焦激光扫描显微镜结合荧光蛋白是研究植物中有性繁殖和其他发展过程的有力工具。 为了理解复杂组织中荧光信号的起源和定位，细胞轮廓的染色通常是强制性的。 最近已经描述了使用SCRI Renaissance 2200（SR2200）的细胞壁染色作为研究植物繁殖过程的选择方法（Musielak等人，2015）。 在该协议中，我们提供关于使用SR2200在不同的拟南芥组织中染色细胞壁的详细说明。
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