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Uranyl Acetate 98%, ACS Reagent

Company: PolyScience
Catalog#: 21447-25
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Transmission Electron Microscopy of Centrioles, Basal Bodies and Flagella in Motile Male Gametes of Land Plants
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2017-10-05
[Abstract]  Motile male gametes (spermatozoids) of land plants are coiled and contain a modified and precisely organized complement of organelles that includes a locomotory apparatus with two to thousands of flagella. Each flagellum is generated from a basal body that originates de novo as a centriole in spermatogenous cell lineages. Much of what is known about the diversity of plant male gametes was derived from detailed transmission electron microscopic studies. Because the process of spermatogenesis results in complete transformation of the shape and organization of these cells, TEM studies have yielded a wealth of information on cellular differentiation. Because green algal progenitor groups contain centrioles and a variety of motile cells, land plant spermatozoids also provide a ... [摘要]  土地植物的运动雄性配子(精子)卷曲,并含有修改和精确组织的细胞器的补体,其包括具有两至数千个鞭毛的运动器官。每个鞭毛都是从起源于新生细胞的基底体产生的,作为精子细胞谱系中的中心粒。关于植物雄性配子多样性的许多知识来自详细的透射电子显微镜研究。由于精子发生过程导致这些细胞的形状和组织的完全转化,TEM研究已经产生了关于细胞分化的大量信息。由于绿藻藻类祖细胞含有中心粒和各种运动细胞,所以土地植物精子也提供了大量的机会来检验土地殖民时期的中心粒和运动器具的演化和最终的损失。
在这里,我们简要概述了我们在过去20年进行的研究和方法,不仅阐明了这些细胞的结构多样性,而且阐明了微管组织中心的发展。 >中心粒的起源和结构复杂的运动细胞的个体发育。
【背景】土地植物的运动配子是非常多样化的,通过涉及重新定位和重塑细胞组分以及组装复杂运动器官的变革来发展(Renzaglia和Garbary,2001; Lopez和Renzaglia,2008)。由于细胞壁施加的限制,细胞和鞭毛的伸长在几乎球形的空间周围,导致成熟配子的盘绕构型(Renzaglia和Garbary,2001; Lopez和Renzaglia,2014)。卷取程度从每个单元只有一到多到十转。每个配子的鞭毛数量甚至更为可变,从苔藓植物(苔藓植物, ...

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