{{'Search' | translate}}
 

Aluminum Slotted Head

Company: Electron Microscopy Sciences
Catalog#: 75220
Bio-protocol()
Company-protocol()
Other protocol()

Scanning Electron Microscopy of Motile Male Gametes of Land Plants
Author:
Date:
2017-10-05
[Abstract]  The only motile cells produced in land plants are male gametes (spermatozoids), which are reduced to non-flagellated cells in flowering plants and most gymnosperms. Although a coiled architecture is universal, the complexity of land plant flagellated cells varies from biflagellated in bryophytes to thousands of flagella per gametes in the seed plants Ginkgo and cycads. This wide diversity in number of flagella is associated with vast differences in cell size and shape. Scanning electron microscopy (SEM) has played an important role in characterizing the external form, including cell shape and arrangement of flagella, across the varied motile gametes of land plants. Because of the size and scarcity of released swimming sperm, it is difficult to concentrate them and prepare them ... [摘要]  在陆地植物中生产的唯一的活细胞是雄性配子(精子),它们在开花植物和大多数裸子植物中被还原成非鞭毛细胞。 虽然盘绕的建筑是普遍的,但是种植植物鞭毛细胞的复杂性在种子植物银杏和苏铁中每个配子的苔藓植物双歧杆菌数量增加到成千上万的鞭毛。 鞭毛数量的多样性与细胞大小和形状的巨大差异有关。 扫描电子显微镜(SEM)在表征陆地植物各种动态配子的外形形态,包括鞭毛的细胞形态和排列方面发挥了重要作用。 由于释放游泳精子的大小和稀缺性,难以集中精力并准备在SEM中观察。 在这里,我们详细介绍了SEM制备技术,可以在植物组中产生精子细胞的良好保存。
【背景】土地植物的运动配子是非常多样化的,通过涉及重新定位细胞组分和组装复杂运动器官的转化来发展(Renzaglia和Garbary,2001)。由于细胞壁施加的限制,细胞和鞭毛的伸长在几乎球形的空间周围,导致成熟配子的盘绕构型。卷取程度从每个单元只有一到多到十转。每个配子的鞭毛数量甚至更多变化,从苔藓植物(苔藓,>随着银杏和苏铁的多样化,剩余的利用花粉管将非活动精子输送到卵细胞的种子植物中,基底体和鞭毛的所有残留都丢失(Southworth and Cresti,1997)。男性配子提供了丰富的生物学信息,包括生物多样性和细胞分化和进化(Garbary et al。,1993; Renzaglia et al。,1995; Renzaglia ...

Comments