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Z-Leu-Leu-Glu-AMC

Company: Bachem
Catalog#: I-1945
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Ubiquitin Proteasome Activity Measurement in Total Plant Extracts
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2017-09-05
[Abstract]  The fine-tuned balance of protein level, conformation and location within the cell is vital for the dynamic changes required for a cell to respond to a given stimulus. This requires the regulated turnover of damaged or short-lived proteins through the ubiquitin proteasome system (UPS). Thus, the protease activity of the proteasome is adjusted to meet the current demands of protein degradation via the UPS within the cell. We describe the adaptation of an intramolecular quenched fluorescence assay utilizing substrate-mimic peptides for the measurement of proteasome activity in total plant extracts. The peptide substrates contain donor-quencher pairs that flank the scissile bond. Following cleavage, the increase in dequenched donor emission of the product is subsequently measured over time ... [摘要]  细胞内的蛋白质水平,构象和位置的微调平衡对于细胞对给定刺激作出反应所需的动态变化是至关重要的。 这需要通过泛素蛋白酶体系统(UPS)调节受损或短寿命蛋白质的周转。 因此,调节蛋白酶体的蛋白酶活性以满足目前通过细胞内的UPS的蛋白质降解的需求。 我们描述了使用底物 - 模拟肽进行分子内淬灭荧光测定来测定总植物提取物中蛋白酶体活性的适应性。 肽底物含有侧向于剪切键的供体 - 猝灭剂对。 切割后,随后随时测量产物的去质子供体发射的增加,并用于计算相对蛋白酶体活性。
【背景】泛素蛋白酶体系统(UPS)是真核细胞中主要的蛋白质降解机制,因此,UPS对许多细胞过程的调节至关重要,包括信号传导,细胞周期,囊泡运输和免疫。用于营业额的蛋白质通过泛素的共价连接标记,然后被26S蛋白酶体降解。 26S蛋白酶体由两个亚颗粒组成,即20S核心蛋白酶(CP),其分隔蛋白酶活性位点和19S调节颗粒,其将适当的底物识别并转移到CP腔中进行分解。调节蛋白酶体活性以维持蛋白酶抑制以响应内部和外部条件的波动。我们最近显示,UPS涉及植物免疫的几个方面,一系列植物和动物病原体颠覆了UPS来增强其毒力(Üstünet al。,2013;üstünet al。,2014;ÜstünandBörnke,2015 ;Üstünet ...

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