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Author:
Date:
2017-08-20
[Abstract] The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability of MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or wall shear stress (WSS), on human MSC immunomodulatory function, we have utilized microfluidics to model WSS at the luminal wall of arteries. Anti-inflammatory potency of MSCs was subsequently quantified via measurement of TNF-α production by activated murine splenocytes in co-culture assays. The TNF-α suppression assay serves as a reproducible platform for functional assessment of MSC potency and demonstrates predictive value as a surrogate assay for ...
[摘要] 认为基于间充质干细胞（MSC）的细胞疗法的有益作用主要是由能够抑制慢性或急性损伤，感染，自身免疫和移植物抗宿主病相关炎症的能力介导的。 为了专门解决由血流或壁剪应力（WSS）引起的摩擦力对人MSC免疫调节功能的影响，我们利用微流体在动脉腔壁上建模WSS。 随后通过在共培养测定中通过活化的小鼠脾细胞测量TNF-α产生来量化MSC的抗炎效力。 TNF-α抑制测定作为MSC效力的功能评估的可重现平台，并且表现出作为MSC治疗功效的替代测定的预测价值。
【背景】间充质干细胞（MSC）的免疫调节活性由直接细胞相互作用和旁分泌因子介导（Singer和Caplan，2011;英语，2013）。 MSCs被认为是源于与骨髓和各种组织内脉管系统内皮细胞相关的周细胞（Sacchetti et al。，2007; Crisan et al。，2008）。这种独特的血管周围位置将它们置于血流中的炎症和其他可溶性因子附近，使其监测系统信号。事实上，将壁壁细胞募集到内皮是血管成熟的关键事件，周细胞在血管维持和完整性中起关键作用（Benjamin et al。，1998; ...
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