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Sputtering coater

Company: EIKO Engineering
Catalog#: IB-2
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Using Silicon Polymer Impression Technique and Scanning Electron Microscopy to Measure Stomatal Aperture, Morphology, and Density
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2017-08-20
[Abstract]  The number of stomata on leaves can be affected by intrinsic development programming and various environmental factors, in addition the control of stomatal apertures is extremely important for the plant stress response. In response to elevated temperatures, transpiration occurs through the stomatal apertures, allowing the leaf to cool through water evaporation. As such, monitoring of stomata behavior to elevated temperatures remains as an important area of research. The protocol allows analysis of stomatal aperture, morphology, and density through a non-destructive imprint of Arabidopsis thaliana leaf surface. Stomatal counts were performed and observed under a scanning electron microscope. [摘要]  叶片上的气孔数量可能受到内在发育规划和各种环境因素的影响,此外,气孔开孔的控制对植物胁迫反应极为重要。 响应于升高的温度,通过气孔开孔发生蒸腾,允许叶子通过水分蒸发而冷却。 因此,将气孔行为监控到升高的温度仍然是研究的重要领域。 该方案允许通过拟南芥叶片表面的非破坏性印记分析气孔孔径,形态和密度。 在扫描电子显微镜下进行气孔计数并观察。
【背景】已经探索了不同的技术来研究各种植物的气孔密度和模式。 它可以大致分为两类,包括直接观察新鲜材料和制备复制品,或铸件(Gitz和Baker,2009)。 这些方法包括使用透明的指甲油,这是用于分析气孔密度的传统方法,然而,叶子的表面可能被指甲油中的溶剂损坏。 在这里,我们使用拟南芥叶片的非破坏性印记进行气孔可视化,其可以直接观察新鲜收集的材料并评估气孔密度用于顺序测量。 此外,它可以简化气孔密度的观察和测量,并且有助于并行分析几条线或不同的处理。

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