| Analysis of Cancer Stromal Reaction Using an O-ring Co-culture Assay
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Author:
Date:
2017-02-20
[Abstract] We have developed a 2D heterotypic co-culture technique between fibroblasts and cancer cells that enables the study of the stromal reaction. For such, stromal cells are seeded and cultured immediately around a tumour cell line, and the cells establish cell-cell contacts, as well as a gradient of soluble factors throughout the stromal cells, similar to that found in tissues. Thus, this system also enables the researcher to distinguish between events that are caused by direct cell-cell contact and secreted factors.
[摘要] 我们在成纤维细胞和癌细胞之间开发了二维异型共培养技术，可以研究基质反应。为此，将基质细胞接种并立即在肿瘤细胞系周围培养，并且细胞建立细胞 - 细胞接触以及遍及基质细胞的可溶性因子的梯度，类似于在组织中发现的。因此，该系统还使得研究者能够区分由直接的细胞 - 细胞接触和分泌因子引起的事件。
背景 组织内肿瘤的生长和存活取决于与周围基质细胞（如成纤维细胞，炎性细胞，内皮细胞和淋巴细胞）的相互作用。研究表明，随着肿瘤生长，癌细胞与周围成纤维细胞之间存在广泛的串扰。此外，肿瘤细胞可以将这些成纤维细胞激活成肿瘤相关成纤维细胞（TAF）。在某些情况下，这些成纤维细胞可能会限制肿瘤生长（Coulson-Thomas等人，2011和2013）;然而，在许多情况下，这些TAF帮助肿瘤细胞生长和存活（Coulson-Thomas等，2010和2015）。因此，体外癌症研究也应考虑到TAF对癌细胞的保护作用。考虑到这一点，我们在成纤维细胞和癌细胞之间开发了2D异型共培养技术，可以在同一系统中研究TAF和癌细胞。
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| Isolation and Primary Culture of Various Cell Types from Whole Human Endometrial Biopsies
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Author:
Date:
2016-11-20
[Abstract] The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then isolated through culturing, filtering and magnetic separation using cell-surface antigen markers. Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments.
[摘要] 从人子宫内膜活组织检查中分离和原代培养细胞为生殖和妇科研究提供了有价值的实验材料。从同意的妇女收集完整的子宫内膜活检组织并用胶原酶和DNA酶I消化以从细胞外基质中分离细胞。然后通过使用细胞表面抗原标记物的培养，过滤和磁性分离来分离细胞群。在这里，我们提供了如何从整个子宫内膜组织分离和培养个体细胞类型以用于体外实验的全面方案。
[背景] 人子宫内膜是子宫内层最粘的层。其由柱状上皮和基础基质层组成，其经历循环再生，生长和响应于循环激素的转化。子宫内膜衬里分化为腺分泌表型为囊胚植入和成功怀孕提供了一个好客的环境。在没有怀孕这个层脱落，导致月经。从人子宫内膜活组织检查中分离和培养细胞允许在体外功能评估和与患者结果相关的细胞特征的研究。子宫内膜细胞的分离和培养是研究妇科和产科医学的许多方面的非常宝贵的研究模型，包括不孕，植入失败，复发性流产和月经紊乱。全人子宫内膜活检包含人子宫内膜基质细胞（HESCs），腔和子宫内膜上皮细胞（HEEC），红细胞和免疫细胞的混合群体。 ...
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| Bone Marrow-derived Endothelial Progenitor Cell Intercellular Adhesion Assay
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Author:
Date:
2016-08-20
[Abstract] Cell-cell adhesion ensures tight contacts between neighbouring cells, which is necessary for cell segregation, as well as for the morphological and functional differentiation of different tissues. Evidently there are cell-cell recognition systems that make cells of the same type preferentially adherent to one another. Homotypic cell adhesion is particularly important in mediating a range of physiological processes such as cell survival, migration and remodeling of vessels. Thus in the present study we selected two populations of endothelial progenitor cells which are from the same donor to investigate the possible effect of a small molecule compound Icariin on homotypic cell adhesion. Many angiogenic factors can destabilize the organization of intercellular junctions, causing endothelial ...
[摘要] 细胞粘附保证了相邻细胞之间的紧密接触，这对于细胞分离以及不同组织的形态和功能分化是必要的。显然，存在使相同类型的细胞优先彼此粘附的细胞 - 细胞识别系统。同型细胞粘附在介导一系列生理过程例如细胞存活，迁移和血管重塑中特别重要。因此，在本研究中，我们选择两个来自相同供体的内皮祖细胞群体，以研究小分子化合物Icariin对同型细胞粘附的可能影响。许多血管生成因子可以破坏细胞间连接的组织，导致内皮屏障开放。在本研究中，我们观察到Icariin治疗减少EPC中VE-钙粘蛋白表达的水平，表明细胞 - 细胞粘附减少 - 证明Icariin的促血管生成作用。总之，所观察到的EPCs的同型粘附的丧失可能有助于由Icariin施加的增强的血管生成作用。
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