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Company: PlumbMaster
Catalog#: 17668
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Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans
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2017-07-20
[Abstract]  Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay is based on the correlation of the rate of neurotransmitter release with the rate of paralysis. In this protocol, we describe a method for simultaneously assessing the aldicarb sensitivity of animals with different genotypes. [摘要]  涕灭威的治疗导致神经肌肉接头突触裂缝中的乙酰胆碱积聚,导致持续的肌肉活化并最终导致麻痹。 涕灭威诱导的麻痹测定是确定是否突触传播的一种简单且快速的方法。 线虫兴趣突变体被改变。 该测定是基于神经递质释放速率与麻痹率的相关性。 在该方案中,我们描述了同时评估具有不同基因型的动物的涕灭威敏感性的方法。
【背景】突触传递通过动作电位到达突触前末端而开始,这又导致神经递质的释放。释放的神经递质结合并激活突触后受体(Sudhof,2013)。 ℃。线虫运动由乙酰胆碱释放兴奋性运动神经元和GABA(γ-氨基丁酸)释放抑制运动神经元控制(Richmond和Jorgensen,1999; ...

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